Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Systems Biology in Reproductive Medicine. All IUI patients had a diagnosis of unexplained infertility. Journal List Transl Androl Urol v. However, the status is that still very few clinics have implemented sperm chromatin integrity testing and therefore most couples seeking help for infertility problems are not aware of their actual potential sperm DNA defects. Journal of Reproduction and Fertility.
Sperm DNA Fragmentation
Gharagozloo P, Aitken RJ The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. This test is an effective method for measuring DNA damage in thousands of sperm in an ejaculate. Initial reports suggest that DNA damage occurs at the post-testicular level, so that testicular sperm may have a healthier DNA integrity than ejaculated sperm 33, Gavriliouk D, Aitken R. Varicocoele is the leading known cause of male infertility and it is associated with sperm DNA damage Other factors include defects in sperm chromatin packaging and DNA repair mechanisms as well as abnormalities in the regulation of programmed cell death abortive apoptosis which is vital for regulating sperm production
Physiology of adult Homo sapiens - Reproductive apparatuses
Syst Biol Reprod Med 57 However, this test does not provide any information about the genetic constitution of the sperm, which is essential for normal embryo development. Normal f ull-term pregnancies are possible with an elevated DFI, but the higher the level of fragmentation, the greater the incidence of reduced term pregnancies and miscarriage. Adv Exp Med Biol One systematic review article suggests that current sperm DNA fragmentation tests have limited capacity to predict the chance of pregnancy following assisted conception treatment 14 , however several large studies, systematic reviews and meta-analyses have revealed that high sperm DNA fragmentation is associated with reduced natural pregnancy rates 6 and assisted conception pregnancy rates 5,6,10,13 as well as live birth rates
Description: The test provides a reliable analysis of sperm DNA integrity that may help to identify men who are at risk of failing to initiate a healthy ongoing pregnancy. Asian J Andrology Thus a high level of DNA damage in sperm cells may represent a cause of male infertility that conventional examinations cannot detect. Reprod Biomed Online Sperm are stained with a fluorescent probe that interacts with the DNA molecule.